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KMID : 1007520170260051357
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Food Science and Biotechnology
2017 Volume.26 No. 5 p.1357 ~ p.1362
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Isolation of thermotolerant yeast Pichia kudriavzevii from nuruk
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Choi Da-Hye
Park Eun-Hee
Kim Myoung-Dong
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Abstract
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Thermotolerant yeast strains were isolated from nuruk, a traditional Korean fermentation starter in which variety of microorganisms are present. Among the isolates, the MBY1358 identified as yeast Pichia kudriavzevii showed significantly higher growth rate (0.59 ± 0.00 1/h) at 44 ℃ than other strains. Maximum ethanol concentration of 8.35 ± 0.03 g/L was obtained from 20 g/L glucose with yield of 0.44 ± 0.01 g/g at 44 ℃, which is 1.14 times ethanol production of the control strain of P. kudriavzevii. The MBY1358, which was significantly more thermotolerant than the control strain and fermented 200 g/L glucose to 107.33 ± 5.03 g/L ethanol at 44 ℃, was deposited to Korean Collection for Type Cultures (KCTC) under the accession number 27654.
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KEYWORD
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Nuruk, Thermotolerance, Yeast, Pichia kudriavzevii, Ethanol
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